Download e-book for kindle: Advances in Low-Temperature Biology, Volume 3 by Peter L. Steponkus

By Peter L. Steponkus

ISBN-10: 0080552285

ISBN-13: 9780080552286

ISBN-10: 0762301600

ISBN-13: 9780762301607

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Additional resources for Advances in Low-Temperature Biology, Volume 3

Sample text

The most significant observation in this Allegheny Phase II study, however, is that whole-body perfusion with the intracellular-type HTS-M solution using precisely the same procedure led to a markedly improved and consistent outcome. , 1995) and warrants repeating in the context of the present discussion. The work of Kondo et al. in 1974 provides a useful reference study for comparison with our experiments since theirs is one of the few studies that has attempted longer than two hours of circulatory arrest using profound hypothermia.

While the dog has been widely used as a pre-clinical model for ^^^* - • , . » - • "^ 30^ »i 1 1 1 25- Z) < 20- »: i ^C * ct: a. '^r ' ^ ^ ^ ^"i7 • • SUBCUTANEpUS _ _ . « • » • - • 5- () 60 120 180 240 300 360 ELAPSED TIME (Minutes) FROM START COOLING Figure 5 420 Hypothermic Protection During Bloodless Surgery 23 40 30 20 10 36- 31- 26- 21- 16- 11-6- 0 6+ 11+ 16+ 21+ 26+ 31+ 36+ 36- 31- 26- 21- 16- 11- 6- 0 6+ 11+16+21+26+31+36+ Temperature range (°C) Figure 5, Mean changes of temperature (A), hematocrit (B), and mean arterial blood pressure (MBP) and heart rate (C) during cooling and warming of dogs in the Phase I studies of ultraprofound hypothermia and blood substitution (UHBS).

These analyses showed that, in general, the UHBS technique led to increased levels of serum enzymes reflecting some perturbation of organ function. The immediate post-operative concentrations of liver, heart, and brain enzymes were statistically higher in dogs from Group I compared with Group II. , 1992). The results of this pilot feasibility study to evaluate a technique of complete blood substitution under conditions of ultraprofound hypothermia clearly demonstrated that the procedure yields an encouraging number of survivors after 2 to 3 hours of hypothermic cardiac arrest.

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Advances in Low-Temperature Biology, Volume 3 by Peter L. Steponkus


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