Read e-book online Advances in Insect Physiology, Vol. 25 PDF

By P. D. Evans

ISBN-10: 0120242257

ISBN-13: 9780120242252

Insect body structure is presently present process a revolution with the elevated software of molecular organic suggestions to enquire the molecular mechanisms underlying the physiological responses to insect cells. Advances in Insect body structure has instituted a dedication to the e-book of top of the range stories on molecular biology and molecular genetics in components the place they supply an elevated realizing of physiological approaches in bugs. quantity 25 comprises elevated assurance at the molecular biology of insect body structure.

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Extra info for Advances in Insect Physiology, Vol. 25

Example text

Like the orthopoxviruses, the EPV virion is brick-shaped or oval with sizes ranging from 150 to 470nm long, and from 165 to 300nm wide. , 1964). , 1971). In cross-section or when the negative stain penetrates the particle, the virion is shown to contain an electrondense core surrounded by a multilayer membrane. EPVs from Orthopteran and Lepidopteran hosts generally contain a cylindrical core and two lateral bodies, while those infecting Dipteran hosts contain a biconcave core and two well-developed lateral bodies.

1992). 4 MOLECULAR STUDIES A restriction enzyme map has been generated for the AmEPV genome using the enzymes HindIII, BamHI and EcoRI (Hall and Hink, 1990), and showed no similarity to restriction enzyme maps of the vertebrate poxviruses. Extensive genomic heterogeneity was detected in the restriction endonuclease cleavage patterns of DNA from five EPVs (EaEPV, MsEPV, Othnonius batesi (Ob)EPV, CbEPV and AmEPV) and vaccinia virus, strain WR (Langridge, 1984). The first gene to be identified and sequenced from an EPV was the putative spheroidin gene from CbEPV (Yuen et a f .

All larval stages, particularly the first two instars, are susceptible to infection (Roberts, 1970), and normal development of the insect during the pupal and imaginal moults is arrested. In Lepidopteran EPV infections, the course of disease is relatively short, usually less than 3 weeks. Infected larvae may become lethargic and lose coordination and mobility during the late stages of infection. Death of E. acrea larvae infected with Amsacta moorei (Am) EPV is frequently preceded by paralysis of the abdomen and by regurgitation or defecation of fluid containing virus.

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Advances in Insect Physiology, Vol. 25 by P. D. Evans

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